1. Introduction

1. Introduction

The greater occipital nerve is located just above the neck on either side of the back of the head. Retinal involvement and intracerebral calcifications aid making the diagnosis. Wrong posture or inadequate exercise is most often the cause of the pain in the lower spinal column. Grab it behind his knees and hip extensor muscles. After the last cortisone injection in January ’09, I am having a genital herpes. But the good news is that chiropractic care is an ideal option for a few seconds on the left side. Please believe me, as I have been through dozens of different medications…(basically became a lab rat for all doctors-including specialists) I definitely know how irritating Erythema can be.

It can enter the moist membranes of the penis, vagina, urinary opening, cervix, or anus. This treatment may in turn, reduce pain, swelling, color and sweating changes in the lower extremity, and may also improve mobility. Varicella-zoster virus and herpes simplex virus types 1 and 2 are neurotropic viruses that can be reactivated after a surgical or stressful intervention. Lumbar pain may often be associated with radiating pain in legs, burning and tingling sensation along the course of nerves. We describe a 41-year-old male with a history of varicella-mediated skin eruptions, who presented with continuing right arm pain, burning, and numbness in a C6 dermatomal distribution following a C5-6 anterior cervical discectomy and fusion and epidural steroid injections. Electrical stimulation of the left LWM at T6 did not evoke any synaptic response in ipsilateral L5 motoneurons of control hemisected animals, indicating interruption of the white matter. Approximately ten days after surgery, the patient presented to the emergency department complaining of severe pain in his right upper extremity and a vesicular rash from his elbow to his second digit.

Here, we apply in EAE mice the gene therapy protocol we have established for IL-4, but using an HSV-1-replication-defective multigene vector engineered with the human fibroblast growth factor (FGF)-II gene (TH:bFGF).17,18 FGF-II is a neurotrophic growth factor able to promote migration and proliferation of oligodendrocyte precursor cells.19 The intrathecal delivery into C57BL/6 mice with ongoing EAE of the TH:bFGF vector determined in situ short-term production of FGF-II leading to amelioration of clinical and pathological signs of EAE via a significant increase of the recruitment of oligodendrocyte precursors into CNS areas of demyelination. Antiemetics – Agents that prevent or alleviate nausea and vomiting. A, Horizontal section of the cord prepared immediately after Hx and immunolabeled with CS-56 showing a low level of CSPGs. This case adds to the limited literature regarding this rare complication, brings attention to the symptoms for proper diagnosis and treatment, and emphasizes the importance of prompt antiviral therapy. Millions of nerve cells situated in the spinal cord itself also coordinate complex patterns of movements such as rhythmic breathing and walking. Varicella-zoster virus (VZV), herpes simplex virus type 1 (HSV-1), and herpes simplex virus type 2 (HSV-2), members of the Herpes virus family, are a group of neurotropic viruses that infect neurons, remain dormant in dorsal root ganglia, and by axoplasmic transport, can become reactivated during times of stress or immunocompromised states in the axonal terminals causing neuralgia, and a cutaneous rash most often in a segmental or radicular distribution. Although afferent sensory neurons are most often affected, motor neuron involvement has been reported in 0.5–31% cases of Herpes Zoster [1].

Thereafter, appearance in the vicinity of the first blister, at margin of the vulva and the anus, of new blisters that the patient comes to show on 17 July. These conditions include certain disorders (such as some cancers, diabetes, and HIV infection), use of drugs that suppress the immune system (immunosuppressants, such as cyclosporine and corticosteroids), and inadequate nutrition. As with spontaneously occurring tumors, these injection-associated sarcomas are amenable to treatment. Of note, after this surgery he experienced a recurrence of his shingles, which subsequently resolved. He presented to our clinic with continued pain and numbness in a C6 dermatomal distribution, as well as a burning sensation in this distribution, despite the previousely mentioned intervention. Berkshire Pain Clinic is the one stop destination for you if you want to get your Back And Spine Pain treated with Invasive Non Surgical Techniques. On physical examination, he demonstrated full strength in all muscle groups bilaterally.

1. Introduction
He had no evidence of myelopathic signs. MRI of the cervical spine upon initial presentation to our clinic showed degenerative disk disease at C5-6, with bilateral foraminal stenosis. Given that he had already undergone attempted foraminal decompression posteriorly, it was decided to proceed with C5-6 anterior cervical discectomy and fusion. Newborn infants can become infected with herpes virus during pregnancy, during labor or delivery, or after birth. THESE ARE THE THINGS DR ERO CAN ALSO CURE.. This was consistent with a herpetic shingles outbreak. They are located in the cervical region, Thoracic region and the lumbar region.

At the follow-up visit, his vesicular rash had completely resolved. Herpes simplex virus infection causes recurring episodes of small, painful, fluid-filled blisters on the skin, mouth, lips (cold sores) , eyes, or genitals. Strengthening and potential. 96 percent of the herpes-filled condoms did not leak the virus. Since 2000 the Joint Commission on Accreditation planning especially your muscles. I know how you feel and I am with you. A positive result confirms the diagnosis, but a negative result does not rule it out.

Patients had resolution of symptoms attributable to Herpes ranging from days to 1 year, with complete to partial resolution of symptoms. In many cases, surgical manipulation of nerve roots led to the development of shingles or herpes radiculitis in dermatomal distribution of the same manipulated nerve roots or those in adjacent levels [4, 5, 7, 9]. Once the course is defined and found as expected, the block medication is injected and the spread is judged with the help of X-rays. The proposed mechanism for symptom resolution is suppression of the intense inflammatory response surrounding the nerves. All images were captured within 48 h of completing the staining procedures. There have been several reported cases where reactivation is manifested after using selective nerve root or epidural injections of steroids [3, 6, 8]. injection of an FGF-II gene-containing HSV-1 vector induces CNS production and secretion of FGF-II into the CSF space from vector-infected cells located around the CSF space.

CRPS – Complex regional pain syndrome, also known as reflex sympathetic dystrophy (RSD) and causalgia, has been recognized for the past 2,500 years and believed in for the past 150, but it has yet to be understood. Additional animals that received hemisection injury and were perfused 20 min (n = 3) or 3 weeks (n = 3) after the injury were used to determine the level of CSPG expression in the vicinity of the injury. Cases with radiculitis have been treated with Vidarabine or IV Acyclovir, and while skin lesions and CSF pleocytosis resolved, neurologic status did not initially change [2, 4]. Severe trauma to the cervical cord results in paralysis of most of the body, including the arms and legs, and is called tetraplegia (though the older term, quadriplegia, is still in common use). Although skin lesions resolved, residual pain persisted at 1-year follow-up [5]. In a case series of postoperative neurosurgical patients who developed VZV or HSV reactivation, oral or IV Acyclovir was used successfully in the resolution of symptoms and lesions [9]. She presents at this time on the left side of the thorax a shingles which extends from the armpit to the centre.

Finally, in the one ophthalmic case encountered in this series and the entire literature review, viroptic ophthalmic solution was prescribed and completely resolved HSV dendritic keratitis in a postoperative neurosurgical patient. Many inactivated vaccines also contain adjuvants or immunopotentiators [168–170]. Gabapentin has shown efficacy in the short-term, that is, less than two months, in preventing postherpetic neuralgia [11] and amitryptiline, which was started immediately after shingles outbreak in conjunction with an antiviral agent in elderly patients at a daily dose of 25 mg has been demonstrated to decrease pain prevalence in patients [12]. Therefore, it was surprising and noteworthy that these agents were not used more frequently in the cases we reviewed. The clinic has expertise in treating a wide range of Pain Conditions through Interventional Procedures, Non Surgical Spinal Interventions, Medication Management and Pain Management & Exercise Programme. In cases with successful resolution, oral or intravenous antiviral agents, most commonly Acyclovir, have been used. Neuromodulating agents such as gabapentin and/or amitriptyline were used in some cases to prevent or decrease the severity of postherpetic neuralgia.

This case report adds to the limited literature regarding this rare complication, brings attention to the symptoms for proper diagnosis and treatment, and emphasizes the importance of prompt antiviral therapy. We suggest the addition of a neuromodulating agent to prevent long-term sequelae and resolve acute symptoms.

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1. Introduction

1. Introduction

Ramsay hunt syndrome[1] is a varicella zoster virus infection of the geniculate ganglion of the facial nerve. After primary infection, it can reactivate with advancing age in immunocompetent hosts, causing the disease herpes zoster. It is also called as herpes zoster oticus. Revered by Ayurvedic practitioners as “the incomparable one,” holy basil has traditionally been used in this ancient system of health and well-being to treat skin conditions, snake bites, ear aches, fevers, coughs, bronchitis, and diabetes. Indications constipation, diarrhea, rectal prolapse, hemorrhoids. Magnetic resonance imaging was performed to rule out other diseases causing diplopia; there were no specific findings other than old infarctions in the pons and basal ganglia. Small lymphocytic lymphoma (SLL), the tissue equivalent of chronic lymphocytic leukemia (CLL), typically presents with lymphadenopathy, organomegaly, and the presence of infiltrating monoclonal B cells having the same immunophenotype as CLL cells but lacking peripheral blood lymphocytosis 1 .

P. Clinically, it manifests as a painless, fluctuant swelling that frequently relapses despite various… This dysregulation of the immune system by mumps virus may have occurred in our patient, enabling the latent VZV infection to reactivate. A variety of virus infections have been linked to the development of peripheral facial paralysis. Occurs more often in the elderly (range of 36 to 83 years of age and a single case of a 9 year old6) with a female predominance.7 The headaches occur only during sleep and awaken the sufferer at a consistent time. Acute Otitis Media: Acute otitis media is an infection of the middle ear and is one of the more common causes of ear pain. The peripheral blood showed a white blood cell count (WBC) of 6.9 × 109/L (4–10.5), neutrophils 4.44 × 109/L (2.00-7.00), lymphocytes 1.89 × 109/L (1.50-4.0), haemoglobin 145 g/L (136–170) and platelets 307 × 109/L (150–400).
1. Introduction

However, the association of other viral infections with the pathogenesis of facial paralysis remains largely unclear because of the paucity of reports. AOE may be idiopathic, secondary to ear canal trauma or related to exposure of the ear canal to water and can therefore be related to swimming. Zoster, meaning belt or girdle in Greek, is felt to be a reactivation of a previous varicella infection within a single dermatome. Daily doses of dapsone can be taken at home by the patient, but monthly doses of rifampicin should be administered by a health worker. This medication usually begins to work within the first few doses. She subsequently developed a right submandibular swelling on the eighth day. Many things can affect the dose of medication that a person needs, such as body weight, other medical conditions, and other medications.

She had been vaccinated against mumps at the age of 2 and had no obvious episode of mumps infection previously. Although she had also been vaccinated for chickenpox at the age of 2, she developed chickenpox at the age of 4. Another past medical history was notable for an operation for Fallot’s tetralogy at the age of 2. At the first visit to our clinic on the tenth day of illness, a physical examination revealed a complete left facial paralysis (House-Brackmann grade VI). A baby whose mother is the genitals or from this situation the human body and in the cheek. Pure tone audiometry showed normal hearing in both ears. Stapedial reflex was absent in the left ear.

The patient also had swelling of the parotid glands bilaterally and the right submandibular gland. Computed tomography images of the neck that had been examined in the pediatric department on the eighth day of illness confirmed sialadenitis of the left parotid gland (Figure 2) and right submandibular gland. We also noted herpes zoster in the left parotid area and posterior cervical area corresponding to the C2-3 area (Figure 3). Next five sittings of block were done with 0.25% bupivacaine only.

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1. Introduction

1. Introduction

The cytoplasmic mRNA which codes for the herpes simplex virus specific thymidine kinase (TK) is polyadenylated and is 14.5s in size. Regarding its polypeptide and glycan structures, as yet there is no detailed insight into their heterogeneity and ligand properties, which may greatly influence its function and biomarker potential. More than half of the genes are dispensable for growth in cell culture and appear to have auxiliary functions that optimize viral replication and spread within its host. tk gene expression was restored by the insertion in 56 of the 67 plasmids analyzed, and the level of expression equaled or exceeded that obtained with the wild-type tk gene in 30 of these. Although BVdU-treated and untreated cells contained similar amounts of virus, very little virus was released into the culture supernatant from BVdU-treated cells. Full Text The Full Text of this article is available as a PDF (334K). These results indicate that a portion of the EHV-1 genome is able to persist in the transformed cells for many generations and be expressed as an enzymatically active viral gene product.

IgG were isolated in 22 cases (7 %) corresponding to previous infection. Mucin 16 (MUC16) is a type I transmembrane protein, the extracellular portion of which is shed after proteolytic degradation. This is denoted as CA125 antigen, a well known tumor marker for ovarian cancer [10–12]. It is placed in the mucin family according to the results of partial cloning of sequence, but due to its specific properties, such as N-glycan composition, MUC16 does not fit well into either class of mucin molecules [13–15]. The type I interferon (IFN-I) system plays a crucial role for vertebrates in controlling viral infections. SEA is an extracellular domain associated with O-glycosylation, which might regulate or assist binding to neighboring carbohydrate moieties [17]. The ankyrin repeats are tandemly repeated modules of about 33 amino acids, which are one of the most common protein–protein interaction motifs [18].

Regarding its polypeptide and glycan structures, as yet there is no detailed insight into their heterogeneity and ligand properties, which may greatly influence the function and biomarker potential of MUC16/CA125 [19–23]. This study was aimed at gaining more insight into the biological capacity of this mucin by exploiting a combination of computational and experimental approaches. Thus, we performed in silico analysis of corresponding mucin sequences, including similarity searches as well as GO (gene ontology)-based function prediction. In the present study, we initially investigated the influence of heparin on infection of ecotropic MLVs including Friend MLV (F-MLV) clone 57 (Oliff et al., 1980) and its neuropathogenic variants, A8 MLV (Takase-Yoden and Watanabe, 1997 and Watanabe and Takase-Yoden, 1995) and PVC-211 MLV (Kai and Furuta, 1984 and Masuda et al., 1992). ED Super Advanced Pack comprises 40 pills – 10 pills each of Viagra 100 mg , Cialis 10 mg, Viagra soft 50 mg and Cialis Soft 20 mg used to improve your performance during your sexual activity. The packaged diluent (Bacteriostatic Water for Injection) contains benzyl alcohol as a preservative. Misra V, Rapin N, Akhova O, Bainbridge M, Korchinski P.

Human and animal herpesviruses are large, enveloped virions with related glycoproteins incorporated into the virion envelope. Journal of Biological Chemistry. Excretion of the administered dose is nearly complete within 12 hours. Seroprevalence studies have indicated that one of two adults in the USA aged 14–49 years old is infected with HSV-1 in a latent state [39]. Cell wall surface anchor family protein (B2ISC7/Q97P71) from Streptococcus pneumoniae and serine-rich adhesin for platelets (Q4L9P0) from Staphylococcus haemolyticus were reported as bacterial hits. GO annotation described these entries as having transmembrane transporter activity and virulence activity mediating binding to specific cells [30–33]. 1, 1993).

Ser/Thr rich regions in high scoring hits, are known to be involved in cell adhesion and pseudohyphal formation or binding to polysaccharides in the natural environment and/or efficient invasive growth on such substrates [34–39]. In addition, the search through the eukaryota database reported mucin-like proteophosphoglycan 5 (E9AEM9) from Leishmania major exhibiting similarity to MUC16. Until this study, it was not known how many of the CHO sites were actually utilized by mammalian cells. The purified PCR fragment was digested with EcoRI and XbaI and ligated into EcoRI/SpeI-digested pET28mod. Our previous results have shown that the interaction of the EHV-1 IE protein with TFIIB is necessary for its full trans-activation function and that the IE-TFIIB interaction may be part of the mechanism by which the IE protein activates transcription (30). Thus, the highest scoring reported candidates are associated with the membrane/cell wall/extracellular region and are involved in different types of adhesion processes based on protein-protein or protein-sugar binding. To prepare strand-specific labeled DNA probes, a small fragment of DNA from one or the other end of the labeled probes was removed by digesting the probes with a restriction endonuclease.

No relation to annotated domains from available databases appeared, except for gp2/BLLF1 (herpesvirus major outer envelope glycoprotein) from conserved domain database (CDD) [42]. Here we have performed a glycoproteomics approach that has enabled a detailed mapping of the order by which individual Ser and Thr residues of the gC-1 mucin domain acquire GalNAc residues. Starting from these observations as well as reported data on elevated CA125 concentration in patients with different type of B cell lymphomas, which could be associated with EBV infection [43,44], anti-human CA125 antibodies were probed for reactivity with herpesviruses glycoproteins. Thus, EBV capsid antigen and HSV 1 antigen were probed with two classes of monoclonal antibodies to MUC16/CA125: OC125/OC125-like and M11/M11-like, reacting mainly with the repeated peptide sequences [13,14,45]. β-Galactosidase activity was quantified using the luminescent β-galactosidase detection kit II (Clontech), following the manufacturer’s instructions. As for HSV 1 antigens, both antibodies gave measurable reactivity, being slightly higher for OC125-like antibody. Generally, there is a phenomenon that unrelated organisms can have antigens in common [46–48].

1. Introduction
Mouse monoclonal anti-human CA125 antibodies: clone X306 (OC125-like) and clone X325 (M-11 like) were allowed to react with immobilized Epstein-Barr Virus (EBV) capsid antigens or Herpes simplex virus type … Accordingly, ORF O and ORF P interfere with the expression of multiple α-regulatory genes which promote lytic gene expression. However, crude yeast extract, as a source of the identified mucin-like molecules, showed no trace of CA125-immunoreactivity (data not shown). The available data indicate that 4% of 600 monoclonal antibodies against a large variety of viruses crossreacted with healthy host tissues and that heterologous immunity may be elicited even by very short common sequences (such as six amino acids) [52]. The biological meaning of such crossreactivity i.e., heterologous immunity, in general, is not understood and also it is not known whether it may have any functional consequences. As part of a strategy for assignment of structural/functional domains, a BLAST search starts with the basic assumption that higher sequence similarity increases confidence in function annotation transfer [53,54]. However, there is no threshold and homology does not always mean similar function.

Thus, in addition to BLAST, protein function prediction software based on GO annotations were also used for computational analysis of CA125 sequence (Tables 2 and 3). Although, the reported matches had low probability scores, they put MUC16/CA125 in the context of modular proteins with an annotated role in adhesion-related processes. In terms molecular function, GO category: binding (GO:0005488) was associated with purine nucleotide (GO:0017076), metal ion/ion (GO:0046872/GO:0043197) or sugar binding (GO:0005529). Mouse anti-Myc, anti-Flag, and antihemagglutinin (anti-HA) monoclonal antibodies (MAbs) were purchased from ABmart (Shanghai, China). In terms of biological processes, GO category: cellular process (GO:0009987) was associated with cell-matrix adhesion (GO:0007160), and GO category: physiological process (GO:0007582) was associated with cell growth (GO:0016049), transport (GO:0006810) and metabolism (GO:0008152). Thus, invasive growth (GO:0001403), cation transport (GO:0006812), i.e., ATP synthesis coupled proton transport (GO:0015986) and polysaccharide metabolism (GO:0000272), were annotated, respectively [57,58]. So far, several lines of experimental evidence obtained on cancer- or pregnancy-associated MUC16/CA125 antigen, indicate possible involvement in adhesive/anti-adhesive processes during cancer progression or embryonic development [59–62].

The precise mechanisms of these processes are not fully explained. Generally, it is suggested that there is link between cell adhesion and ion transport. For instance, local extracellular pH levels at tumor focal adhesion sites modulate the strength of cell adhesion i.e., more protons leads to tighter adhesion and decreased migration [63]. These processes can involve different molecules, but there are data substantiating the existence of adhesion molecules with amino acid identity (40%) and immunologically cross-reactive to the beta subunit of Na/K-ATPase [64]. With increasing doses of corticosteroids, the rate of occurrence of infectious complications increases. 1996. It is known that mucins as ligands for cell-cell adhesion molecules (CAM) or as CAM themselves are an important part of the adhesion interaction network based on carbohydrate-binding interactions.

Kowalski, P.J. With increasing doses of corticosteroids, the rate of occurrence of infectious complications increases. Specifically, the modified vaccinia virus Ankara (MVA) has been used to express HSV-2 gD, inducing strong cellular and humoral immunity [143]. On the other hand, MUC16 was reported to be localized on the surface of uterodome (pinopode) protrusions of the endometrium, acting as a barrier for trophoblast adherence [62]. Cell-matrix contact structures, i.e., cellular protrusions can be morphologically different, but mechanisms of spreading are thought to be similar in normal and pathologically altered cells [66]. cited by applicant . MUC16 has a distinct evolutionary relationship with other transmembrane mucins.

Using sequence comparison of well characterized mucin domains: SEA, NIDO, AMOP and VWD, it was shown that MUC16 evolved separately, before the divergence of birds and mammals [67]. Hybridomas secreting anti-gH MAbs MP6, MP7, and MP8 were generated based on previously described methods (7) with purified gH from HSV-1-infected cells (36). The protein was over 95% pure as determined by Coomassie brilliant blue staining (Fig. The 0.66-kb EcoRI and SacII fragment of pSVICP0K was cloned into the EcoRI and SacII sites of pUC19SII to generate pUEICP0(1-202). The protein sequence of human mucin 16 (MUC16) (ovarian cancer-related tumor marker CA125), accession number Q8WXI7 (UniProtKB, The Universal Protein Knowledgebase), accession number IPI00103552 (IPI, The International Protein Index), accession number NP_078966.2 (NCBI refSeq, National Center for Biotechnology Information Reference Sequence) was retrieved from public databases [68,69]. ). The following protein knowledgebases were searched: bacteria, viruses, fungi, eukaryota; and the highest scoring candidates were ranked under different parameter settings (threshold, matrix, filtering, gapped sequence).

and mounted with Prolong Gold Anti-fade containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen). Mouse monoclonal anti-human CA125 antibodies: clone X325 (M-11 like) and clone X306 (OC125-like) were from HyTest (PharmaCity, Turku, Finland). They were allowed to react with immobilized Epstein-Barr Virus (EBV) capsid antigens (CA), from Epstein-Barr Virus (EBV) VCA IgG kit (Virion/Serion GmbH, Wurzburg, Germany), or Herpes simplex virus type 1 (HSV 1) cell culture-derived antigens, from Herpes simplex virus type 1 IgG kit (Human GmbH, Wiesbaden, Germany). H22 and Y26, while not part of the motif, are residues that are conserved among several members of this family of endonucleases and have been proposed to play a role in substrate specificity (14, 20, 21). Subsequent to incubation for 1 h, the wells were rinsed and Vectastain Elite ABC reagent (Vector Laboratories, Burlinghame, CA, USA) was added followed by incubation for 30 min. After another washing step, addition of TMB substrate solution and incubation for 10 min, the reaction was stopped with 0.16 M H2SO4. The absorbance was measured at 450 nm on a Wallac 1420 Multilabel Counter (Monza, Italy).

, lane 4) which contains a deletion in the TK gene, and with an α27 promoter-driven TK gene replacement of the 1,100-bp StuI-NcoI fragment encoding the ORF P and γ134.5 genes. Serial dilutions of crude yeast (Saccharomyces cerevisiae) extract in 0.01 M carbonate buffer, pH 9.2, were adsorbed on polystyrene test tubes (Spektar, Cacak, Serbia) overnight at 4 °C. The tubes were then rinsed three times with 0.1 M PBS, pH 7.2, blocked with 1% casein for 2 h at RT and rinsed again three times with 0.1 M PBS, pH 7.2. Reaction with the corresponding monoclonal anti-human CA125-antibodies was then allowed as described for viral antigens. Since protein function has many facets and is highly contextual, bioinformatic data on the predicted GO molecular function of CA125 can be considered in the light of possible general principles shared across distant distinct, yet related proteins. The results obtained suggested a possible correlation between the role of the serine/threonine rich domain of yeast, acting as a sensor for extracellular osmotic pressure and that of the mucin domain of transmembrane mucins in monitoring extracellular ion gradients and pH [35,79,80]. In addition, a possible relationship has emerged between mucin participation in polarized growth and directional motility i.e., amoeboid mechanisms of propulsion and mucin-like fungal proteins in pseudohyphal and filamentous growth involving sugar-substrate binding [81,82].

CA125-immunoreactivity of Herpesvirus antigens. Mouse monoclonal anti-human CA125 antibodies: clone X306 (OC125-like) and clone X325 (M-11 like) were allowed to react with immobilized Epstein-Barr Virus (EBV) capsid antigens or Herpes simplex virus type 1 antigens (HSV1). (A) Schematic diagram of construction of the C40A HSV-1 BAC. The absorbance was measured at 450 nm. Non-specific binding was estimated using an irrelevant monoclonal anti-hCG antibody (c).

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