Hello all. Any reactive donation is discarded per local and state regulations. Patients and Methods Samples were obtained from patients enrolled in AIDS Malignancy Consortium clinical trials and from healthy donors. Still other medications are perfectly fine, like allergy medicines, birth control shots, and antacids. The websites at the end of this topic also include information about eligibility criteria, which may vary slightly between different blood centers (see ‘Where to get more information’ below). I read that one can get back aches if they have herpes. Medical history interview — All blood donors are asked questions about their medical history to help determine whether they can safely donate blood without experiencing any negative health effects .
Median copy numbers also differed. Chronic allergy sufferers, or those taking strong doses of anti-histamines are disqualified. People with heart disease, heart valve conditions, irregular heartbeat, disease of the blood vessels in the brain, heart failure, and certain lung conditions may be excluded from blood donation, or they may be allowed to donate blood provided this has been cleared with their healthcare provider and they have had no major symptoms in the prior six months. But, after reading the bach ache part, I have been a little paranoid. A prospective donor should mention other serious medical conditions to the donor health historian who will then evaluate eligibility to donate. KSHV DNA in plasma likely reflects viremia and not simply lysis of tumor or other KSHV-infected cells. Taking blood thinners while donating leads to abnormal plasma, clotting, excessive bleeding, and unacceptable quality plasma for making anti-anemic medications.
●Age requirement – The minimum age for blood donation is 16 or 17 years, depending upon the state. When allowed, 16-year-olds must bring a signed permission form from a parent. In most cases, there is no upper age limit for donation, although approval from the donor’s physician is required in some cases. Blood specimens were obtained from patients with untreated, aggressive, B-cell AIDS-NHL enrolled in an AIDS Malignancy Consortium trial.10 Pretreatment blood specimens from patients with AIDS-KS entered on two AIDS Malignancy Consortium trials11,12 and from healthy blood donors (screened to eliminate HIV-seropositive donors and those with evidence of other infection that would preclude blood donation) were also studied. Anticonvulsant (Anti-Seizure) Medication: you must wait 1 year after your last seizure before donating again. Although reactions to blood donation are rare, individuals weighing between 50 and 54 kg (110 and 119 pounds) are most likely to experience reactions. Most blood centers perform an additional evaluation of donors aged 16 to 18 who are just over the weight limit, which takes into account the donor’s estimated blood volume, calculated from the donor’s height and weight.
There is no upper weight limit for donating blood, although some centers have an upper weight limit based on the size/strength of the donor phlebotomy chair. DNA was isolated by using the QIAGEN Blood Kit (QIAGEN Inc, Valencia, CA). Asthma Medication: must wait 30 days after last athsma attack to donate, cannot donate if you’ve ever been in the ER for an athsma attack. Individuals with a fever, high blood pressure (generally higher than 180/100), very high or very low heart rate (with the exception of highly conditioned athletes and those on beta blocker medication), or an irregular heartbeat are temporarily not permitted to donate blood. Time interval until next donation — Donors are eligible to donate no sooner than 56 days (eight weeks) after their previous donation. However, this minimum interval can vary, depending upon how rapidly the person’s body is able to replenish its red blood cells. Real-time primers and a probe for β actin were used as controls in some experiments.
Cholesterol Medication: high cholesterol controlled under medication, you may donate plasma. Many blood organizations recommend that, in addition to eating foods rich in iron, people who donate this frequently take a multivitamin with iron or an iron-only supplement to replace the iron lost through each blood donation. A technology called apheresis has made it possible to collect specific components of blood. Apheresis is used to selectively collect red blood cells, platelets (blood components that play an important role in clotting), plasma, and granulocytes (a type of infection-fighting white blood cell). Virions for use as controls in DNase protection assays were prepared from JSC-1 cells.13 TPA was added to achieve a final concentration of 20 ng/mL in culture. After 1985, taking growth hormones results in no disqualification. It takes from one to two hours.
●Red blood cells – Donors can give red blood cells by apheresis once every 16 weeks. This is less frequent than whole blood donation because a greater amount of red blood cells are collected during the apheresis procedure. The pellet was resuspended at 4°C overnight. Sleeping Pills and Tranquilizers: you can donate immediately, with no waiting period. The reaction can be treated or prevented by taking a calcium supplement before or during the donation. ●Granulocytes – Donors of granulocytes may be given granulocyte colony-stimulating factor (G-CSF) and/or a glucocorticoid medication called dexamethasone on the day before donation to increase the number of granulocytes in their blood. Glucocorticoids are usually not given to individuals who have diabetes, gastrointestinal ulcers, or glaucoma.
Similarly, none of the patients had a diagnosis of primary effusion lymphoma or other KSHV-associated lymphoproliferative disease. Generally, clinical trial drugs have to be out of your system for 30 days before plasma donating. A variety of different measures, including self-reporting of infectious exposures and other infectious disease risk factors, and laboratory testing of the blood, are used for this screening. ●Elimination of payment for donation – Since the late 1970s, volunteer donors have been the source of all whole blood and blood components in the United States. Donors are sometimes paid for donating plasma that is used to manufacture other blood products. KSHV copy numbers in PBMCs and in plasma did not correlate with HIV RNA levels, absolute CD4 counts, or CD19 counts (Appendix Table A1, online only). Human immunodeficiency virus (HIV) — The human immunodeficiency virus (HIV) is the virus that causes AIDS.
A variety of measures are used to screen donors for HIV infection or risk factors for HIV, including questions about any signs or symptoms of HIV and behaviors that increase the risk of HIV. Such behaviors include using non-prescription intravenous (IV) drugs, having sex with a prostitute or in exchange for money or drugs, and for men, having sex with another man. Depending on your answers to these questions, you may not be allowed to donate blood, or you may need to wait for a period of time before you are eligible. Copy numbers are shown as the log10 of copies per million PBMCs or per 100 μL plasma. People who want to be tested for HIV or other sexually transmitted infections should not donate blood for this purpose. Even though donated blood is tested for HIV, the tests are not perfect and there is a small possibility that a person who might have been exposed to HIV could transmit the virus to a recipient if they donate blood. Free and anonymous HIV testing is available elsewhere (see www.hivtest.org).
●People who have ever had a positive test for hepatitis B surface antigen (a marker for hepatitis B virus infection) are permanently disqualified from donating blood. Whiskers show the 10th to 90th percentiles. In order to avoid being disqualified as a blood donor, a person who has recently received the hepatitis B vaccine should wait 21 days before donating blood. At that time, this test should be negative. ●People who have had sexual contact or have lived in the same dwelling (eg, house, dormitory) with someone who has hepatitis (A, B, or symptomatic hepatitis C) are disqualified for 12 months after their last exposure to that person, depending upon the particulars of the exposure. ●In some states, people who have received a tattoo are not permitted to donate blood for 12 months. DNase digestion.
With regard to ear or body piercing, donation is allowed if the procedure was performed with sterile or single-use equipment. ●Chagas disease and babesiosis – Transmission of Chagas disease (American trypanosomiasis) by transfusion is rare in the United States. Blood donors are asked if they have ever had Chagas disease, and most donated blood is tested for Chagas disease. Prion diseases — Creutzfeldt-Jakob disease (CJD) is a rare but fatal neurologic disease. (B) Cell DNA from a cell line that harbored KSHV episomes. Affected individuals may have no symptoms of CJD or vCJD for many years. The infectious agent of both these diseases is an abnormally folded protein called a prion.
Bacterial infection — Bacteria can get into donated blood if a donor has a bacterial infection, if bacteria on the skin gets into the blood from the needle stick, or if there is a skin infection near the location where the blood is drawn. To reduce the likelihood of bacterial contamination of blood, the skin around the site is carefully examined and cleaned before the needle is inserted. The standard curves for each amplicon are shown in Figure 3A. These restrictions also apply to individuals who are banking blood for their own use (see ‘Autologous blood donation’ below). Other infections — Donated blood cannot be tested for every possible infection, and new types of infections are frequently emerging around the world. Examples of such infections are Ebola virus and Zika virus. The pre-donation educational material given to donors, as well as the questionnaire, includes several general questions that are designed to identify individuals who either have infection symptoms or who have travelled to locations where these infections are more common (eg, possible exposure to Zika virus due to travel to certain countries).
Both large fragments and small fragments were present in plasma, which is consistent with the notion that KSHV is in virion particles. However, questions in the pre-donation questionnaire are directed at general signs of infection, and individuals who might be at risk of transmitting an infection due to travel or behaviors are asked to “self-defer,” which means to avoid donating blood. The decision to self-defer can be made at the time of donation or after the donation has been completed (in which case the center will not use or distribute the donated blood). ●Cancer – There have been no reported cases of the transmission of cancer by blood transfusion. However, because such transmission is theoretically possible, donors are screened for a history of cancer. Follow-up specimens were evaluated for all patients in whom pretreatment study showed KSHV detection as well as for 10 patients in whom KSHV was not detected in pretreatment specimens. This time period varies but is at least one year.
Donors who have had a superficial cancer that has been completely removed by surgery (such as basal cell cancer of the skin or early cervical cancer) can donate blood without any waiting period. ●Hemochromatosis – Individuals with hereditary hemochromatosis (a condition in which frequent removal of blood is the standard treatment) can donate their blood for transfusion if they meet other criteria for being a donor and if the blood bank has met certain regulations from the US Food and Drug Administration (FDA). The acceptance of blood donations from these individuals has not been uniformly implemented, perhaps due to the costs of meeting regulations and removing financial incentives to blood donation. Peripheral-blood mononuclear cell (PBMC) specimens from patients with AIDS–non-Hodgkin’s lymphoma (NHL) before the initiation of combination chemotherapy and before follow-up specimens were assayed for Kaposi’s sarcoma herpesvirus (KSHV). Recent vaccinations — There are strict standards for when you can donate blood after you have received a vaccination. When you go to donate blood, you will be asked if you have had any recent vaccinations. If you have, you might have to postpone your blood donation.
For some live or attenuated (weakened) viral or bacterial vaccines (measles, mumps, oral polio, oral typhoid, yellow fever), you have to wait at least two weeks before donating your blood. X axis, relationship to the initiation of chemotherapy as measured in weeks; * patient treated with CHOP alone who had residual CD19+ cells detected at the first assay point after lymphoma chemotherapy was initiated. Donors who have taken isotretinoin and finasteride are asked to wait one month after the last dose before donating blood; donors who have taken dutasteride are asked to wait six months; donors who have taken vismodegib are asked to wait seven months; and donors who have taken acitretin are asked to wait three years. Individuals who have taken etretinate are permanently disqualified from donating blood. People who took aspirin or aspirin-containing medications within the previous 48 hours are allowed to donate whole blood but are not allowed to donate platelets by apheresis. People who took anti-platelet drugs (eg, clopidogrel [sample brand name: Plavix] or ticagrelor [sample brand name: Brilinta]) must wait a variable period ranging from 2 to 14 days to donate platelets by apheresis. This finding—that, among AIDS patients with detectable KSHV DNA in plasma, KSHV copy number did not differentiate patients with KS from those with NHL—suggests that the KSHV DNA being measured in plasma is unlikely to be tumor-derived, even in patients with KS.
Anti-clotting medicines include warfarin (sample brand name: Coumadin), dabigatran (brand name: Pradaxa), rivaroxaban (brand name: Xarelto), apixaban (brand name: Eliquis), and edoxaban (brand names: Savaysa, Lixiana). The reason for this is that the person who receives the blood may get some of the effect of the blood thinner. Confidential unit exclusion (CUE) — The confidential unit exclusion (CUE) process allows someone who has donated blood to confidentially indicate that his or her blood should not be given to others. This process protects individuals who feel pressured to donate in the workplace or during community blood drives. Our finding of a high fractional concentration of KSHV fragment sizes in plasma larger than 180 bp also suggests an origin other than release of free DNA from cells undergoing apoptotic cell death. This is done after the interview, but before donation. Although this procedure was commonly used in the past, most United States blood banks no longer use it.
Registry of deferred donors — A registry of deferred donors contains names of individuals who have been disqualified from blood donation in the past. Some donors in the registry have infectious diseases, such as hepatitis B or HIV infection. Thus, our results confirm the observations already reported by others. Other donors in the registry have provided information in the past that disqualified them from blood donation. A donor’s name is usually checked against this registry before and after donation. The reason for the deferral is not usually available to staff at the collection facility. Telephone callbacks — After donating blood, donors are given a phone number so that they can call the donation center to report any factors that may affect the use of their blood or to report symptoms of infections in the first days to two weeks after donating (such as symptoms of upper respiratory tract infection, gastrointestinal illness, or infection with a virus transmitted by mosquitos).
It has been suggested that lymphoid tissue sustains KSHV viremia and that B cells are the lymphoid reservoir.31 We have previously reported the rapid decline (within 24 hours) of EBV viral copy number in PBMCs of patients with post-transplant lymphoma who were treated with rituximab alone.32 This corresponds to a disappearance of B cells from blood that accompanies rituximab treatment. Autologous blood donation is when a donor donates blood for her or himself several days to six weeks ahead of a scheduled surgery, when blood might be needed. Autologous blood donation reduces the risk of most, but not all, infectious complications of blood transfusion. A small percentage (2 to 5 percent) of people feel faint and/or pass out before, during, or after donating blood. This is more common the first time a person donates and in people who are younger. Alternatively, virions produced elsewhere might be carried in cell-free blood to precursor cells. This article will be updated as needed on our web site (www.uptodate.com/patients).
Related topics for patients, as well as selected articles written for healthcare professionals, are also available. Some of the most relevant are listed below. The Basics — The Basics patient education pieces answer the four or five key questions a patient might have about a given condition. Standard lymphoma chemotherapy with CHOP or CHOP-R led to rapid declines in viral DNA in PBMCs and in plasma. Beyond the Basics — Beyond the Basics patient education pieces are longer, more sophisticated, and more detailed. These articles are best for patients who want in-depth information and are comfortable with some medical jargon. Professional level information — Professional level articles are designed to keep doctors and other health professionals up-to-date on the latest medical findings.
These articles are thorough, long, and complex, and they contain multiple references to the research on which they are based. Professional level articles are best for people who are comfortable with a lot of medical terminology and who want to read the same materials their doctors are reading.