We present the locations of the cleavage sites for the BamI, KpnI, and SalI restriction endonucleases within the DNA molecules of herpes simplex virus type 1 (HSV-1) strains Justin and F. Nasal septa tissue is the major site of wt viral replication post intranasal (i.n.) inoculation. Immunocytofluorescence analysis with antibodies to HSV-1 revealed that PACT eliminated HSV-1 and ACV-resistant HSV-1 in a manner dependent on the TONS 504 concentration and light energy. Reported here are experiments performed to determine whether the reduced reactivation of KOS was associated with a reduced ability to establish or maintain latent infections. For comparative purposes, latent infections were quantified by (i) quantitative PCR on DNA extracted from whole ganglia, (ii) the number of latency-associated transcript (LAT) promoter-positive neurons, using KOS and 17syn+ LAT promoter-beta-galactosidase reporter mutants, and (iii) contextual analysis of DNA. By placing windows around the 272 breakpoints followed by Monte Carlo analysis comparing actual data to simulated data, we identified a recombination bias toward both high GC content and intergenic regions. We applied this approach to quantify the amount of variation between clonal derivatives of a common parental virus stock.
No correlation was detected between the syncytial phenotype and local vaginal virulence. Nat. Acad. Thus, cleavage of these regions with restriction endonucleases yielded sets of minor fragments differing in size by constant increments. U.S.A.69, 3784–3789 (1972). Halliburton, I. Mailing address: University of Cincinnati Medical Center, Department of Molecular Genetics, Microbiology, and Biochemistry, 231 Bethesda Ave., Cincinnati, OH 45267-0524.
In:Biggo, P. Herpes simplex virus 1 (HSV-1) is a double-stranded DNA (dsDNA) virus in the Alphaherpesvirinae subfamily which causes recurrent, mucocutaneous lesions. We used this approach to quantify the amount of variation between sister clones of a common parental virus stock and to determine the basis of a unique fusion phenotype displayed by several variants. (eds.), Oncogenesis and Herpesviruses, 432–438. Lyon, France: International Agency for Research on Cancer 1972. Hayward, G. S., Frenkel, N., Roizman, B.: The anatomy of herpes simplex virus DNA: strain difference and heterogeneity in the location of restriction endonuclease cleavage sites.
Proc. Nat. Acad. Sci. It is likely that homologous recombination resolves double-strand breaks and assists in the formation of Y-junction origins of replication. Hayward, G. S., Jacob, R.
J., Wadsworth, S. C., Roizman, B.: Anatomy of herpes simplex virus DNA: Evidence for four populations of molecules that differ in the relative orientations of their long and short segments. Proc. Nat. Acad. Sci. U.S.A.72, 4243–4247 (1975).
Cells.To generate viral DNA stocks, green monkey kidney (Vero) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 5% serum and antibiotics as described previously (33). W., Roizman, B.: Proteins specified by herpes simplex virus. IX. Identification and relative molar rates of synthesis of structural and non structural herpes virus polypeptides in the infected cells. J. Virol.12, 1347–1365 (1973). Pereira, L., Cassai, E., Honess, R.
W., Roizman, B., Terni, M., Nahmias, A.: Variability in the structural polypeptides of herpes simplex virus 1 strains: potential application in molecular epidemiology. Infect. Immun.13, 211–220 (1976).