Herpes viruses are involved in a variety of human disorders. A Triton X-100 extract of herpes simplex virus type 1 (HSV-1) infected HEp-2 cells was used to coat wells of polyvinyl chloride plates. Two oyster herpes-like virus specific primer pairs, A5/A6 and C1/C6, were used. We also report evidence by in situ hybridization that these latently infected ganglia contain HSV-1 RNA homologous to the BamHI SP region of the genome which is transcribed in the same direction as the other latency transcripts. If these two specimens were considered to be true positive, Herpchek, IDEIA HSV and SureCell HSV assays had a sensitivity of 88.7 %, 83.0 % and 47.2 %, and a specificity of 100 %, 97.9 % and 85.1 % respectively. During latency, staining with anti-ICP-4 was detected in 14 out of 18 ganglia tested; 10-15% of the ganglion cell nuclei per section exhibited positive staining. PCR significantly increased HSV detection in both early (MagNA Pure LC >QIAamp DNA mini kit (table 3).
The ability of the defined in situ hybridization technique to diagnose herpes-like virus infections in oysters was compared with light and transmission electron microscopy techniques in infected and non-infected spat. Terms Related to the Moving Wall Fixed walls: Journals with no new volumes being added to the archive. Absorbed: Journals that are combined with another title. Complete: Journals that are no longer published or that have been combined with another title.