Cytoplasmic localized infected cell protein 0 (bICP0) encoded by bovine herpesvirus 1 inhibits β interferon promoter activity and reduces IRF3 (interferon response factor

Cytoplasmic localized infected cell protein 0 (bICP0) encoded by bovine herpesvirus 1 inhibits β interferon promoter activity and reduces IRF3 (interferon response factor

Previous studies have suggested an important role of the cytokine adjuvant IL-6 in the induction of mucosal immune responses in animals, including mice. BoHV-5 is the causal agent of non-suppurative meningoencephalitis in calves. Transfection of the restriction enzyme-excised, linear E3-deleted BAV3 genomic DNA into primary fetal bovine retina cells produced infectious virus (BAV3.E3d), suggesting that all the E3-specific open reading frames are nonessential for virus replicationin vitro. There was a 93.6 per cent prevalence of indirect immunofluorescent antibody to BHV-3 in the sera of 94 buffaloes in the sample population. The infected cell protein 0 (bICP0) encoded by BHV-1 reduces human β-interferon (IFN-β) promoter activity, in part, by inducing degradation of interferon response factor 3 (IRF3) and interacting with IRF7. In contrast to humans, cattle contain three IFN-β genes. BAV3 and BHV1 antigens were determined to be 5.1 % and 1.7 % in sheep and 0.7 % and 2.2 % in goats by the IP method, respectively.
Cytoplasmic localized infected cell protein 0 (bICP0) encoded by bovine herpesvirus 1 inhibits β interferon promoter activity and reduces IRF3 (interferon response factor

In latently infected cattle, the latency-related (LR) RNA is the only abundant transcript that is expressed. The most prominent histopathological findings were massive degeneration, necrosis and erosions of the lining epithelium of the alimentary tract. In this study, we demonstrate that bICP0 effectively inhibits bovine IFN-β promoter activity following transfection of low passage bovine cells with interferon response factor 3 (IRF3) or IRF7. BoHV-4 replicates in animal cell lines like Georgia bovine kidney cells, baby hamster kidney cells, Crandell feline kidney cells, and equine dermal cells as well as in human cell lines like human embryonic lung cells and HeLa cells (16, 39). Most importantly, the available vaccine strains, like the wild-type virus, may down-regulate the cell surface expression of major histocompatibility complex class I molecules (12, 23), which likely compromises the development of cytotoxic T lymphocytes against not only BHV-1, but also other viruses and intracellular pathogens. Although these vectors are effective with respect to the entry and expression processes, expression of the transgene is transient (8). BoHV-1 also initiates shipping fever, a potentially fatal polymicrobial disease (37).

The lungs and liver were routinely cultured for common aerobic bacterial pathogens. ► A viral encoded or induced function promotes shuttling of bICP0 from cytoplasm to nucleus during productive infection.

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