Equine Herpesvirus 1 Multiply Inserted Transmembrane Protein pUL43 Cooperates with pUL56 in Downregulation of Cell Surface Major Histocompatibility Complex Class I

Equine Herpesvirus 1 Multiply Inserted Transmembrane Protein pUL43 Cooperates with pUL56 in Downregulation of Cell Surface Major Histocompatibility Complex Class I

Equine Herpesvirus 1 Multiply Inserted Transmembrane Protein pUL43 Cooperates with pUL56 in Downregulation of Cell Surface Major Histocompatibility Complex Class I
Equine herpesvirus 1 (EHV-1) is a member of the Alphaherpesvirinae, and its broad tissue tropism suggests that EHV-1 may use multiple receptors to initiate virus entry. You can find out more about our use of cookies in About Cookies, including instructions on how to turn off cookies if you wish to do so. The diagnostic performance of the new technique was evaluated by testing specimens collected from 234 horses involved in recent outbreaks of EHV-1 myeloencephalopathy at three separate thoroughbred racetracks and one large riding/boarding stable. At the indicated times following infection, supernatants and cell pellets were harvested for determination of extracellular and intracellular titers, respectively. For addressing the more dynamic aspects of PBMC-EC interaction, infected PBMC were perfused through a flow channel containing EC in the presence of neutralizing antibodies. Persistence of the expression of latency-associated transcripts in NS, as a reflection of a latent viral state, was not documented during the 28-day study period. In addition, ODA is working to notify owners of horses that have been potentially exposed and has notified Oregon equine veterinarians.

Rick Arthur has been Equine Medical Director for the California Horse Racing Board since 2006. Thus, despite its common occurrence in several horse breeds, Eqca-1*00101 is associated with a narrow binding repertoire and a similarly narrow T cell response to an important equine viral pathogen. All experiments were independently performed in triplicate and analyzed by using the Student t test. Data are presented as means ± standard deviations (error bars). At present, there is no vaccine available in Ethiopia, and therefore, outbreaks of EHV-1 should be controlled by proper management adaptations. The incubation period of EHV-1 is typically 2 to 10 days.

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Equine Herpesvirus 1 Multiply Inserted Transmembrane Protein pUL43 Cooperates with pUL56 in Downregulation of Cell Surface Major Histocompatibility Complex Class I

Equine Herpesvirus 1 Multiply Inserted Transmembrane Protein pUL43 Cooperates with pUL56 in Downregulation of Cell Surface Major Histocompatibility Complex Class I

Equine Herpesvirus 1 Multiply Inserted Transmembrane Protein pUL43 Cooperates with pUL56 in Downregulation of Cell Surface Major Histocompatibility Complex Class I
Subcellular localization of pUL43 and role of TM domains at the C terminus. (A) Putative structures of pUL43 and its mutant lacking the N-terminal 40 amino acids. At 6 h p.i., cells were analyzed by flow cytometry after incubation with mouse anti-MHC-I (CZ3) MAb and Alexa Fluor 647-labeled goat anti-mouse IgG. Moreover, mutational analysis revealed that an RSD motif in the EHV-1 envelope glycoprotein D (gD) is critical for entry via endocytosis. (B) Lysosomes rather than proteasomes are responsible for degradation of pUL43. The recent upsurge in the number of large, high-mortality outbreaks of equine herpesvirus-1 (EHV-1) neurological disease poses an emerging threat to equine health and to the economic prosperity of horse-related businesses.2,4,7,9 Rapid, laboratory-based diagnosis of the disease is important for prompt implementation of infection-control measures designed to minimize transmission of the virus to other horses at the outbreak site. Individual viruses were used to infect RK13 cells at an MOI of 0.0001 and overlaid with methylcellulose.

Taking the results together, we conclude that systemic spread and EC infection by EHV-1, but not EHV-4, is caused by its ability to infect and/or reprogram mononuclear cells with respect to their tethering and rolling behavior on EC and consequent virus transfer. To detect proteins, cell lysates were prepared in RIPA buffer. It is a common virus and may lie dormant for long periods of time, then re-activate during a period of stress, which can result in clinical disease. He chaired the racing committee of the American Association of Equine Practitioners and served on the Quality Assurance Program of the Racing Commissioners International. β-Actin was included as a loading control. Molecular mass markers were run for each blot in parallel, and sizes are indicated in kilodaltons.

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