Expression and purification of Suid Herpesvirus-1 glycoprotein E in the baculovirus system and its use to diagnose Aujeszky’s disease in infected pigs

Expression and purification of Suid Herpesvirus-1 glycoprotein E in the baculovirus system and its use to diagnose Aujeszky’s disease in infected pigs

Expression and purification of Suid Herpesvirus-1 glycoprotein E in the baculovirus system and its use to diagnose Aujeszky’s disease in infected pigs
Renault Tristan, Allain Gwenhael, Arzul Isabelle, Chollet Bruno, Cobret Laetitia, De Decker Sophie, Faury Nicole, Ferrand Sylvie, Francois Cyrille, Garcia Celine, Haffner Philippe, Joly Jean-Pierre, Miossec Laurence, Morga Benjamin, Nicolas Jean-Louis, Omnes Emmanuelle, Pepin Jean-Francois, Saulnier Denis, Schikorski David, Segarra Amelie (2009). A PHV-1 specific indirect enzyme linked immunosorbent assay (ELISA) was developed to sequentially measure the serological status of 106 harbor seal neonates admitted to a Pacific coast rehabilitation center (total number of sera tested was 371). DNA from HSV-1, EBV, CMV and HHV-8 was detected in 8 (33%) of the sinus aspirates from HIV-infected patients. Expression of the HHV-8-specific proteins at very high levels by the rSFV system allowed easy scoring for IFA and thereby increased specificity. As previous efforts to clone the gE gene had failed due to its extremely high GC-content (75% average), we used betaine as a PCR enhancer to facilitate amplification of the entire gE gene from the Argentinian CL15 strain of SHV-1. (ii) The synthesis of α polypeptides did not require prior infected cell protein synthesis. CpHV.1 establishes latent infections, but, unlike other herpesviruses, its reactivation is extremely difficult to demonstrate under both natural and experimental conditions and very rarely has been reported (1, 4, 8).

For the most frequently found herpesvirus, HHV-6A/B, we examined its association with sperm. Funding: Filomena Fiorito was supported by a fellowship from Polo delle Scienze e delle Tecnologie per la Vita dell′Universitá di Napoli “Federico II” (2012-4/STV-Progetto FORGIARE) co-funded by Compagnia San Paolo di Torino, Italy.

You may also like