Eight patients with brain biopsy-proven herpes simplex encephalitis were seen at the Massachusetts General Hospital, Boston, within a three-month period in the summer of 1977. The sequence of herpesvirus Ig-like receptor (HIgR) predicts a transmembrane protein with an ectodomain consisting of three cysteine-bracketed domains, one V-like and two C-like. Nucleotide sequencing revealed that the gB BHV-2 gene is highly conserved compared to gB-1. Landry, W.P. Two further open reading frames (ORF) in close vicinity to the gene encoding gB BHV-2 showed considerable homology to HSV-1 genes. The blue markers indicate the concentration of mutant DNA (copies/µl) and the green markers indicate the concentration of wild-type DNA (copies/µl) in each of three replicate samples. Berkovitz and Dr Summers), Human Genetics (Dr.
In situ hybridization analysis with probes that can detect all three transcripts shows that the RNAs are detectable in a large fraction of BCBL-1 cells prior to lytic induction and in >70% of KS spindle cells in primary KS tumors. Summers), and Neurology (Dr. Of these four membrane-associated proteins, only UL20 and gK are essential for the intracellular transport of virions to extracellular spaces in all cell types (6, 16, 18, 22, 24). Every HSV-1 sample from the United States except one matched the European strains, but one strain that was isolated in Texas looked Asian. The low rates of HIV suggest that the intervention was started before this population became sexually active. The three brain isolates, the isolate from the nurse, and several epidemiologically unrelated strains were analyzed by restriction endonuclease mapping. All were determined to be distinct strains of herpes simplex virus.
The possibility that a single strain of virus caused this cluster of cases was therefore examined directly and disproved.