A robot-assisted automated facility was established within a biosafety level 3 laboratory located on a university campus. Special sections include articles on antimicrobial resistance, bioterrorism, emerging infections, food safety, hospital epidemiology, and HIV/AIDS. Microsporidia emerged as important opportunistic pathogens in humans when AIDS became pandemic and, more recently, have also increasingly been detected in otherwise immunocompromised patients, including organ transplant recipients, and in immunocompetent persons with corneal infection or diarrhea. Intramuscular (IM) or intradermal (ID) immunization in mice elicited antibodies to gB that were relatively stable over time and predominately of the IgG2a isotype. 9, 439–447 (2009). Among the nonhuman-primate herpesviruses, only herpes B virus is clearly able to cause disease in humans. Since that time, over 200,000 tests have been performed to identify BV infected monkeys and humans.
Note: In calculating the moving wall, the current year is not counted. Infection with B virus is extremely rare in humans. Das Herpesvirus simiae, auch Herpesvirus B, B-Virus oder laut ICTV Cercopithecines Herpesvirus 1 (CeHV-1) genannt, ist ein bei Altweltaffen der Familie Cercopithecidae (Meerkatzen, Paviane und besonders Makaken) vorkommendes Virus der Gattung Simplexvirus. Sci. J. This overall workload with potentially hazardous reagents and primate sera justified investment in an automated system for handling the laboratory’s routine tasks. (2003) 77(11): S.
15 Herpes B (Herpesvirus simiae) AGENT: DNA Herpesvirus. First reported case of encephalitis caused by B virus in monkey handler was in 1932. 50, 1436–1443 (2005). Guidelines for prevention of Herpesvirus simiae (B virus) infection in monkey handlers. To the best of our knowledge this is the first published report on a fully automated serological laboratory in an academic facility. Clinical: Tuberculosis in monkeys, especially rhesus, is a rapidly progressive disease, and seldom becomes arrested as in humans. Transmission: fecal-oral, rodent feces most common source.
Mother usually clinically normal. 37, 1481–1489 (2003). History: This monkey was challenged with simian immunodeficiency virus (SIV) approximately 4. (NC) Genesis 150 liquid handler that was custom integrated into the BECKMAN-COULTER SAGIAN Core System by BECKMAN-COULTER (IN). Herpes B, an alpha-herpesvirus, is a common virus of rhesus macaques that can cause lesions on the dorsal surface of the tongue and at the mucocutaneous junction; however, the gross lesions are generally only associated with an acute primary infection and are rarely noted in chronically infected animals (6). Candida is a part of the normal flora of the oropharynx and gastrointestinal tract, and so is a common source of secondary infections. The zoonotic potential of herpes B virus and simian parvovirus are probably more important than their potential impact on drug safety studies (Brown et al.
28th Ed. B Virus (herpes B, monkey B virus, herpesvirus simiae, and herpesvirus B). The eight tips were used to dispense 15μl, 50μl and 100μl water per well to three separate plates. An average of over 35,000 cases were reported during thistime period. Clinically indistinguishable from polio paralysis but twice as deadly, the incidence of NPAFP was directly proportional to doses of oral polio received. Macaques with oral lesions suggestive of B virus infection should be quarantined until the lesions have healed to reduce the risk of virus transmission to workers and other macaques. Am.
Cultures of oral and conjunctival specimens were performed every 3 to 4 days. Two basic programs, one for automatic coating of ELISA plates and the second for the automation of the titration ELISA (tELISA) were written using the SAMI NT (Beckman-Coulter) and the Gemini (TECAN) softwares. B-virus, Herpesvirus simiae: historical perspective. H. simiae latent in rhesus and other macaques, fatal in man (also known as Herpes B Virus). Enferm. Herpesvirus B was first described in 1934 by Sabin/Wright.
All antigens were adsorbed in the presence of 60% picric acid.