A new in vitro system based on real-time PCR was developed for evaluation of human herpesvirus 8 susceptibility to antiviral agents. The prognosis is poor, with reported median survival time shorter than 6 months. We show that the HHV-8 TK is a functional deoxythymidine (dT) kinase, with Michaelis constants (Km) for dT and ATP of 18.5 and 6.6 μM, respectively. Samples were analyzed at the start of highly active antiretroviral therapy (HAART) and at different intervals during treatments. The absolute number of expressing cells was inducer and cell line dependent. HHV-8 has also been correlated with body cavity-based lymphomas (6) and multicentric Castleman’s disease (37). NF-AT and NF-κB activation by vGPCR was greatly increased by the HIV-1 Tat protein, although Tat alone had little effect on NF-AT.
Enveloped viruses have evolved two main pathways to mediate their entry into the cells after attachment to cell surface moieties (reviewed in reference 25). However, HHV-8 can also be spread through saliva and blood which is harder to prevent . If you are in contact with someone with HHV-8, it is best to be cautious and to understand the risks if you come into contact. Therefore, study of the HHV-8 cytokines is relevant to understanding the mechanisms by which HHV-8 induces neoplasia and for developing therapeutic interventions. Of importance is the normal appearance of the most superficial epidermal layer on the far left, which was overlying the dermal layer, which contained a cellular infiltrate, and a proliferation of vascular elements.” This image is from the CDC website.