In vivo small animal PET/CT imaging of herpes simplex virus type 1-thymidine kinase expression by human telomerase reverse transcriptase RNA targeted trans-splicing ribozyme

In vivo small animal PET/CT imaging of herpes simplex virus type 1-thymidine kinase expression by human telomerase reverse transcriptase RNA targeted trans-splicing ribozyme

In vivo small animal PET/CT imaging of herpes simplex virus type 1-thymidine kinase expression by human telomerase reverse transcriptase RNA targeted trans-splicing ribozyme
The essentiality of HSV-1 UL7 in viral replication in cell cultures has been controversial (Roizman & Knipe, 2001), and no experimental evidence supporting the assumptions of essentiality has been reported. BVDU and its congeners were much more inhibitory to the growth of FM3A/TK-/HSV-1 TK+ than to the growth of the wild type (FM3A/0) cells. By continuing to browse this site you agree to us using cookies as described in About Cookies. Methods Adenoviral vector (AdCRT) expressing the trans-splicing ribozyme was constructed. coli and partially purified and their enzymatic properties compared. It was also shown that intranuclear aggregates, presumably of nonspecific protein, were responsible for the granular appearance of the inclusion body, and that the Cowdry type A inclusion body was formed by fusion of these aggregates. In HCT-116 cells expressing the Glu-125 TK mutant, GCV metabolism was greatly attenuated, yet at higher GCV concentrations, cell sensitivity to the drug and bystander effect killing were diminished but still effective.

Results 125I-FIAU uptake was about 8-fold increased in hTERT positive cells (19.64±0.31%) compared with hTERT negative cells (2.56±0.42%) after AdCRT infection. Specific uptake of 124I-FIAU was observed in hTERT positive tumors through in vivo PET/CT imaging as well as biodistribution analysis. Conclusions This study showed specific HSV1-tk expression using hTERT RNA targeted trans-splicing ribozyme in hTERT positive tumor. Short Title: Herpes simplex NOS 054.

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