Jason S Buhrman | College of Pharmacy

Jason S Buhrman | College of Pharmacy

A phase II trial evaluating combination of radiotherapy and an experimental treatment driving tumor cells to self-destruct achieved promising results in cancer regression and presented no indication of tumor recurrence. A number of projects in our laboratory are focused on the prevalence of KSHV infection in various cohorts and populations. Theoretically, combination therapy could potentially allow reovirus to target drug or radiotherapy resistant subpopulations of tumour cells, or chemotherapy could be utilised to improve the biodistribution of oncolytic viruses. Aggressive and non-aggressive tumours were compared. This effect was associated with altered expression and transcriptional activity of the androgen receptor (AR). NV1042-treated mice exhibited a transient increase of serum IL-12 1 day posttreatment, whereas IL-12 levels in tumor bearing lungs persisted a further 2 days at least. However, only Adcav-1tk produced significant necrosis, and only Adcav-1tk and AdRSVtk caused significant decreases in microvessel density.

ACS Biomaterials Science and Engineering. Further examination of our data failed to show any difference in the prevalence of staining for HSV-2 based on whether the source of the tissue was surgical or autopsy. Therefore, to further inform the possible relation between HHV-8 infection and PCa, and to investigate the reproducibility of previous suggestive inverse findings, we conducted a case-control study sampled from the placebo arm of the Prostate Cancer Prevention Trial (PCPT). Causes for the racial disparity in prostate cancer incidence are unknown. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. In addition, only ECE-1b and 1c proteins were detected in primary stromal cells, with increased levels in the malignant state when compared to benign.19 Furthermore, ECE-1c is also reported by Ahmed et al.20 as the most abundant isoform in lung cancer cells. 2015;275(3):746-54.

doi:10.1148/radiol.14140251. Rayahin JE, Buhrman JS, Gemeinhart RA. “Melittin-glutathione S-transferase fusion protein exhibits anti-inflammatory properties and minimal toxicity”. Amplification and detection of HPV on all samples was carried out using the PapType High-Risk (HR) HPV Detection and Genotyping kit (Genera Biosystems, Melbourne, Victoria, Australia) [17]. 2014;(65):112-121. doi:10.1016/j.ejps.2014.09.012. COPD Prostate Cancer Clinical Trials.

a total of 1,054 urine, 150 serum, and 150 prostate tissue samples from patients with PCa or BPH were used in the study. Hum Gene Ther 23: 871-882. 2014;(58(1)):83-90. doi:10.1016/j.peptides.2014.06.008. Of 240 samples that were available for C. For the comparator traditional whole cell vaccine, cells were resuspended at a concentration of 5 106 cells in 200 l of PBS, placed in a sterile container and irradiated 50 Gy. Journal of Controlled Release.

After 3 minutes of denaturing at 95°C, 35 cycles of denaturing for 40 seconds at 95°C, annealing for 35 seconds at 52.5°C, and extending for 45 seconds at 72°C were performed in a Programmable Thermal Controller. doi:10.1016/j.jconrel.2013.06.023. Other elements indicated are the ampicillin resistance gene (amp), origin of replication (ori), SV40 (SvpA), bovine growth hormone (BGHpA) polyadenylation regions, SAT gene, and RSV promoter. “Active, soluble recombinant melittin purified by extracting insoluble lysate of Escherichia coli without denaturation”. P, positive control for PCR using 10 ng of plasmid pBStk; N, negative control for PCR without DNA. The concentration of DC-Chol was adjusted to 1 mg/ml in the final nanoparticle suspension with water. doi:10.1002/btpr.1784.

Jason S Buhrman | College of Pharmacy
Zhang W, Fulci G, Wakimoto H, Cheema TA, Buhrman JS, Jeyaretna DS, Stemmer Rachamimov AO, Rabkin SD, Martuza RL. “Combination of oncolytic herpes simplex viruses armed with angiostatin and IL-12 enhances antitumor efficacy in human glioblastoma models.”. Neoplasia (New York, N.Y.). 2013;15(6):591-9. Köllmer M, Buhrman JS, Zhang Y, Gemeinhart RA. “Markers are shared between adipogenic and osteogenic differentiated mesenchymal stem cells”. Radiation may improve transduction efficiency and transgene integration, while drugs that act on DNA synthesis (introduced via enzyme/prodrug gene therapy strategies) may increase the susceptibility of the tumour to the cytotoxic effects of radiotherapy.36 Preclinical studies have shown that multimodal treatment may have a synergistic effect on treatment efficacy when compared with the effects of either treatment modality alone, in an animal prostate cancer model.37 A clinical trial is in progress to examine the combination of HSV TK suicide therapy with conventional radiotherapy.36 It is feasible that a role in localised therapy may be established in the relatively near future.

In this study, the recombinant adenovirus was constructed to replicate in PSES-positive cells, which express both PSA and PSMA (). doi:10.5897/JDBTE2013.0065. To purify the His-tagged eIF-2α protein, a His•Bind purification Kit (Novagen Inc., Madison, WI) was used following the manufacturer’s instructions. To assess the AP activity of ADV-rPB-DR(6)-AP in the primary cultures of human normal nonprostate cells, ADV-rPB-DR(6)-AP or ADV-CAG-AP was used to infect smooth muscle cells, hepatocytes, and human umbilical vein endothelial cells (HUVECs). We compared these cases both to controls in the nested case-control study and to all at-risk PLCO participants in the intervention arm. After excluding nine papers [55-63] with no information about HPV type 16 among the 17 papers present in the EXP list but absent from the A group, eight papers [64-71] were added to the B group. doi:10.1186/1472-6750-12-63.

Rayahin JE, Buhrman JS, Gemeinhart RA. Following vaccine administration, dendritic cells are recruited to the site where antigen can be processed and presented to T lymphocytes in the local lymph nodes. Expert Opinion on Therapeutic Patents. Although CMV seropositivity is generally believed to be stable over time, it is possible that, in men with borderline CMV seropositivity, recent (i.e., close in time to visit 2) reinfection with a different CMV strain or reactivation may lead to both a rise in CMV antibody levels at visit 2 and either infection-mediated PSA elevation or infection-mediated prostate abnormalities at visit 2 or subsequent visits, as PSA has been found to remain elevated for at least six months following sexually transmitted infectious episodes (20). doi:10.1517/13543776.2012.665877. Zhang W, Fulci G, Buhrman JS, Stemmer-Rachamimov AO, Chen JW, Wojtkiewicz GR, Weissleder R, Rabkin SD, Martuza RL. “Bevacizumab with angiostatin-armed oHSV increases antiangiogenesis and decreases bevacizumab-induced invasion in U87 glioma.”.

Molecular therapy : the journal of the American Society of Gene Therapy. 2012;20(1):37-45. doi:10.1038/mt.2011.187. Kleijn A, Chen JW, Buhrman JS, Wojtkiewicz GR, Iwamoto Y, Lamfers ML, Stemmer-Rachamimov AO, Rabkin SD, Weissleder R, Martuza RL, Fulci G. “Distinguishing inflammation from tumor and peritumoral edema by myeloperoxidase magnetic resonance imaging.”. HSV-1 γ34.5 mutants cannot inhibit this response, limiting their growth in many cell types. 2011;17(13):4484-93.

The TERE1 gene, for example, seems to play a role in prostate cancer progression through a growth regulatory pathway, possibly in G1 phase of cell cycle. Castelo-Branco P, Passer BJ, Buhrman JS, Antoszczyk S, Marinelli M, Zaupa C, Rabkin SD, Martuza RL. In a series of papers Livingston and her colleagues all continued important cancer microbe research showing the characteristic “connective tissue parasite” of cancer, the germ that could be found inside the cell (intracellular) and outside the cell (extracellular) in all cancers they studied. Gene therapy. 2010;17(6):805-10. doi:10.1038/gt.2010.20. Passer B , Castelo-Branco P, Buhrman JS, Varghese S, Rabkin SD, Martuza RL.

“Oncolytic herpes simplex virus vectors and taxanes synergize to promote killing of prostate cancer cells.”. Cancer Gene Therapy. 2009;16(7):551-560. Passer BJ, Castelo-Branco P, Buhrman JS, Varghese S, Rabkin SD, Martuza RL. “Oncolytic herpes simplex virus vectors and taxanes synergize to promote killing of prostate cancer cells.”. Following trypsinization, RM-9 cells were counted and resuspended in HBSS. 2009;16(7):551-60.


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