Multiplex-PCR and oligonucleotide microarray for detection of eight different herpesviruses… – Abstract

Multiplex-PCR and oligonucleotide microarray for detection of eight different herpesviruses... - Abstract

Multiplex-PCR and oligonucleotide microarray for detection of eight different herpesviruses... - Abstract
Human herpesvirus-6 (HHV-6) is the causative agent of the common childhood infectious disease, exanthem subitum. In this study, we report microRNAs of two related herpesviruses, infectious laryngotracheitis virus (ILTV) and herpesvirus of turkeys (HVT), as well as additional MDV2 microRNAs. OBJECTIVE: The artesunate sensitivity of HHV-6 was analyzed and compared to that of several other human herpesviruses. Consequently, researchers have a very detailed picture of the genetic content of herpesviruses and their relationships to each other. However, in case of viral diseases the development of antivirals from natural source is less explored probably because within the virus there are few specific targets where the small molecules can interact to inhibit or kill the virus. Both EBV and HHV-8 have evolved specific mechanisms in order to: (1) interact with the B cell developmental machinery; (2) allow infected B cells to escape from the control of the immune system; (3) affect the B cell cycle checkpoints; (4) mimic and influence B cellular proliferation and differentiation pathways. In total, 227 specimens were tested including 23 CSF, 10 whole blood, 73 plasma, 10 proficiency-testing samples and 111 negative control samples.

Kaposi sarcoma tissue served as a positive control for HHV-8, and EBV-associated lymphoma served as a positive control for EBV. Microarray detected 10 possible double and one triple infection. Negative control samples (70 serum, 30 CSF and 11 proficiency-testing samples) were all negative.

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