ND10 protein PML is recruited to herpes simplex virus type 1 prereplicative sites and replication compartments in the presence of viral DNA polymer…

ND10 protein PML is recruited to herpes simplex virus type 1 prereplicative sites and replication compartments in the presence of viral DNA polymer...

ND10 protein PML is recruited to herpes simplex virus type 1 prereplicative sites and replication compartments in the presence of viral DNA polymer...
The DNA synthesized in herpes simplex virus (HSV)-infected nuclei in vivo was analyzed by chromatography on benzoylated naphthoylated DEAE-cellulose columns. Under these conditions the rapid first-order foldback reaction occurred. We chose the DNA polymerase from phycodnavirus (which infects microalgae) as the basis of this analysis, as it represents a virus of a primitive eukaryote. Entry into the nucleus is also achieved by many viral proteins (reviewed by Fulcher and Jans, 2011), which either assist in viral replication or are required for the formation of progeny viral subparticles or capsids. (Some do contain or produce essential enzymes when there is no cellular enzyme that will serve.) When a complete virus particle (virion) comes in contact with a host cell, only the viral nucleic acid and, in some viruses, a few enzymes are injected into the host cell. The 11 plaque-purified viruses analyzed from an antibody-resistant population obtained from one animal corresponded to four different mutant genotypes, although their consensus sequence remained wild type. Adenovirus E4orf 3 gene deletion mutants retain ND10 and begin replication at the peripheries of ND10.

We also found that the intercalating drug quinacrine significantly inhibited icp35 IRES activity in vitro and reduced the mortality rate and viral copy number in WSSV-challenged shrimp. Campadelli-Fiume G., Arsenakis M. Get a printable copy (PDF file) of the complete article (2.4M), or click on a page image below to browse page by page. In wild-type infection, stage III cells are quickly replaced by cells containing replication compartments (stage IV). PML and ICP8 staining are both observed within replication compartments, indicating a potential role for PML in HSV-1 replication. Models for the role of ND10 proteins in the formation of replication compartments are discussed.

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