Plasma membrane topology of syncytial domains of herpes simplex virus type 1 glycoprotein K (gK): the UL20 protein enables cell surface localizatio… –

Plasma membrane topology of syncytial domains of herpes simplex virus type 1 glycoprotein K (gK): the UL20 protein enables cell surface localizatio... -

One of the most common forms of keratitis is produced by the herpes virus. Chaperone proteins often facilitate the folding and multimerization of such complex structures. gB and gH/gL execute fusion. Microsequencing proved that this protein is the lymphocyte-specific tyrosine protein kinase p56lck. These sets yielded exclusively ori+L viral progeny. The results were confirmed by serological investigation. Additionally, one of the isolates showed a mixed cleavage pattern of 1a and 1b.
Plasma membrane topology of syncytial domains of herpes simplex virus type 1 glycoprotein K (gK): the UL20 protein enables cell surface localizatio... -

G. Herpes of the eye, or ocular herpes, is caused by the herpes simplex virus. Virol. Natl. To determine whether gK is expressed on cellular surfaces, as well as its membrane topology, we generated the recombinant viruses gKV5DI, gKV5DII, gKV5DIII, and gKV5DIVcontaining insertions of the V5 antigenic epitope within each of four domains of gK predicted to localize either in the cytoplasmic side or in the extracytoplasmic side of cellular membranes. Immunohistochemical and confocal microscopy analyses of infected cells showed that both wild-type and syncytial forms of gK were expressed on cell surfaces. Analysis of the topology of the V5-tagged gK revealed that gK domains I and IV were located extracellularly, whereas domains II and III were localized intracellularly.

Transiently expressed gK failed to localize in cellular plasma membranes. In contrast, infection of gK-transfected cells with the gK-null virus DeltagK enabled expression of gK on cell surfaces, as well as gK-mediated membrane fusion. HSV Type 1 causes cold sores and can affect the face and eyes.

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