Qualitative and quantitative analysis of human herpesviruses in chronic and acute B cell lymphocytic leukemia and in multiple myeloma

Qualitative and quantitative analysis of human herpesviruses in chronic and acute B cell lymphocytic leukemia and in multiple myeloma

The UL97 protein of human cytomegalovirus (HCMV, or HHV-5 (human herpesvirus 5)), is a kinase that phosphorylates the cellular retinoblastoma (Rb) tumor suppressor and lamin A/C proteins that are also substrates of cellular cyclin-dependent kinases (Cdks). However, in many cases, no infectious agent can be demonstrated, and these clinical diseases rapidly progress to death. Marek’s disease is a highly contagious neoplastic poultry disease of major economic significance worldwide. Swabs taken from different lesions were used for infection of Vero cells and cythopathic effect was observed. Both rabbits died after a short period of hospitalization. The viability of HHV-1-infected porcine PBMCs was significantly lower than that of mock-infected cells. This study shows that LHV-4 has the smallest simplexvirus genome characterized to date.
Qualitative and quantitative analysis of human herpesviruses in chronic and acute B cell lymphocytic leukemia and in multiple myeloma

With the patients’ and donors’ informed consent, a total of 101 BM or blood samples were tested for HHV loads. Blood and BM samples of 61 patients with B cell malignancies (29 B-ALL, 13 children and 16 adults; 21 B-CLL; 11 multiple myeloma) were collected at diagnosis prior to treatment. Fusion of the viral envelope with the plasma membrane of the cell requires the other four glycoproteins (gD, gB and the gH-gL complex), with the binding of gD to one of its cell surface receptors being an essential event for herpesvirus entry, targeting a series of interactions between gB and gH-gL that occurs concurrently with fusion [31]. This finding documents for the first time the perinatal transmission of a specific HHV-8 subtype. Non-purified samples were submitted to Ficoll centrifugation to separate mononuclear cells, then kept frozen in liquid nitrogen until use. For B-ALL, B-CLL and AML patients, most samples contained at least 90% of tumor cells (blasts for B-ALL and AML, B lymphocytes for CLL). For MM patients, only samples with at least 50% plasma cells were collected.

Moreover, HHV-6 may act as a cofactor to accelerate progression of human immunodeficiency virus disease. Unlike the telomeric termini with perfect telomeric hexameric repeats, the subtelomere region has complex degenerate repeat structures, genes and gene expression, together with active recombination [12,13,14]. Pellets of BM or blood mononuclear cells (aliquots of 3 to 5 millions cells, except for aliquots of purified B lymphocytes or CD34+ cells, which contained 1.5 to 2 millions cells) were thawed, washed in PBS and genomic DNA was prepared using QIAamp DNA mini-kits for blood and tissue samples (Qiagen, Valencia, CA, USA). We found that the beta and gamma CHPKs, but not the alpha CHPKs, share at least a subset of activities with the cellular cyclin-dependent kinases (Cdks) that are key components of cell cycle regulation. The etiology of AE is not known, and most patients do not have obvious precipitating factors. The complete MeHV-1 genome encodes 79 putative genes [12].

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