To compare the SmartCycler II system (Cepheid, Sunnyvale, Calif) results with those of standard cell culture, to compare the SmartCycler II system results with those of a dedicated polymerase chain reaction facility, and to establish the SmartCycler II system as a polymerase chain reaction method for detecting viral and chlamydial DNA from ocular specimens. Three of 10 DNAs extracted from Kaposi sarcoma biopsies contained DNA sequences homologous to radioactively labelled human CMV DNA probe. Upon admission, the patient presented with pain, leg paresis, and urinary incontinence, as well as pleocytosis in the CSF. Three clinical isolates showed mutations leading to amino acid alterations: one had a mutation of K122 to N, which is a gG-1–to–gG-2 alteration; another contained all mutations which were observed in KOS 321 except two silent mutations; and the third isolate carried five missense mutations. The family Asfarviridae contains a single double-stranded DNA (dsDNA) virus called African swine fever virus (ASFV), which is thought to have evolved from an ancestral virus common to the nucleocytoplasmic large DNA viruses including poxviruses, iridoviruses, and phycodnaviruses (10, 11). Replication of both the wild-type and a LAT-negative mutant virus was suppressed in primary embryonic fibroblasts obtained from LAT-expressing transgenic mice compared to that in cells obtained from normal mice. 35 to 98% of wild-type levels.
These results demonstrate that the viral TK gene is required for the efficient establishment of latency. There was no history of underlying immunodeficiency. Two of 109 HSV-2 culture-negative specimens tested positive in the PCR assay (specificity, 98.2%). The forward primer sequence was 5′-AGCCGAAAGGATTCCACCAT-3′ (base pairs 47287 to 47306, Genbank accession U75698).