Regulation of cellular genes transduced by herpes simplex virus.

Regulation of cellular genes transduced by herpes simplex virus.

Herpes simplex virus (HSV)-derived vectors are currently being developed for the introduction of foreign DNA into neurons. Using the lacZ reporter gene fused in-frame with ICP6 regulatory sequences to assay expression quantitatively, we showed that the ICP6 promoter responded very weakly to the alpha-transinducing factor (TIF) in the absence of all other viral gene products, but much more strongly to immediate early proteins. Here we present an analysis of the herpes simplex virus 2 (HSV-2) Us2 ortholog and demonstrate that, like PRV Us2, HSV-2 Us2 is a virion component and that, unlike PRV Us2, it does not interact with ERK in yeast two-hybrid assays. The “moving wall” represents the time period between the last issue available in JSTOR and the most recently published issue of a journal. Of the 12 open reading frames that were mutagenized with Tn5, mutant derivatives of US2, US4, and US5 were recombined into the virus. No single subclone or pair of subclones demonstrated significant inhibition of transient gene expression. Virol.
Regulation of cellular genes transduced by herpes simplex virus.

In contrast, AN was either missing or present at only low levels in each of these structures. We report here that the beta-globin promoter remained under early control after insertion into the late HSV gene encoding glycoprotein C. Absorbed: Journals that are combined with another title. We found that a transduced human alpha-globin gene was also regulated as an early HSV gene, while two linked Alu elements mimicked the behavior of HSV late genes. These results demonstrate that certain aspects of HSV temporal regulation can be duplicated by cellular elements and provide strong support for the hypothesis that the regulation of HSV gene expression can occur through mechanisms that do not rely on recognition of virus-specific temporal control signals. Full text Full text is available as a scanned copy of the original print version. Tegument proteins enter the cell upon fusion of the viral envelope with the host cell membrane and therefore can exert their activities prior to viral gene expression.

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