Replication Characteristics of Herpes Simplex Virus Type-1 (HSV-1) Recombinants in 3 Types of Tissue Cultures

Replication Characteristics of Herpes Simplex Virus Type-1 (HSV-1) Recombinants in 3 Types of Tissue Cultures

Replication Characteristics of Herpes Simplex Virus Type-1 (HSV-1) Recombinants in 3 Types of Tissue Cultures
Purpose: : Successive acyclovir (ACV) treatment of patients with recurrent herpes simplex virus type 1 (HSV-1) keratitis (rHK) may induce ACV resistance. You can find out more about our use of cookies in About Cookies, including instructions on how to turn off cookies if you wish to do so. Links to PubMed are also available for Selected References. Here, we provide a research update about the interaction of HSV with DC and summarize the latest proceedings in this field. Using homologous recombination in cell culture, the recombinants were constructed and their DNA were analyzed by Southern-blotting. In addition, for survivors, days of mechanical ventilation (8 +/- 7 versus 2 +/- 4, p = 0.03), length of stay in the intensive care unit (10 +/- 8 versus 2 +/- 4 days, p = 0.004), and length of stay in the hospital (37 +/- 23 versus 16 +/- 14 days, p = 0.04) were all significantly longer for the nonimmunocompromised patients. No trigeminal ganglion latency was detected after inoculation with either HSV-2 isolate.

The results showed that (i) ICP34.5 restored the ability to replicate and prevent host shutoff similar to wild type in SK-N-SH cells (ii) ICP34.5 restored the replication phenotype to near wild type levels in 3T6 cells and (iii) ORF P expression had no effect on the replication of these mutants. The characteristics of 1716 is obviously due to the lack of ICP34.5 and ORF P has no role in the characteristics studied. Additionally, this study builds upon previous observations that the viral immediate-early protein ICP0 plays a role in reducing the levels of heterochromatin associated with the early genes.

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