During HSV-1 infection, IE gene expression triggers apoptosis, but subsequent synthesis of infected cell proteins blocks apoptotic death from ensuing. Subjects who the investigator believes that they can and will comply with the requirements of the protocol; Male and female subjects at least 18 years old at the time of vaccination; Serological evidence of prior VZV infection for all subjects born in 1980 or later and for subjects born outside the US before 1980 in a tropical or sub-tropical region. The question is could this be early signs of herpes, all the internet sites say 2 to 20 days for symptoms. And only some of the couples in the study used condoms. For instance hypothetically if you slept with a man and 2-3weeks later you had an outbreak when going to the doctors if it was herpes the swab culture would come back positive and a blood test done at the same time more then likely would come back negative…in that situation you would know it was definitely recently acquired. The research team will collect the data 1 month in average after the initial visit by examining the microbiologic data of the laboratory. In our center, the flocked swabs became an important tool in the corneal sampling for viral keratitis, replacing rightly or wrongly the former traditional fibers swab.
No research was specifically designed to study the corneal taking with these swabs. Nevertheless, numerous reasons would motivate it, because of the peculiarities in ophthalmology: the corneal sampling has to be made with the slit lamp rather than in a macroscopic manner; the corneal lesion are often millimetre-length and confined; the histology of the cornea is unique and compares only imperfectly with other structures of the human body like the nasal mucosa. For example, during latency, the LAT regions of the LAT reactivation-critical promoter and LAT 5′ exon (containing the LAT enhancer) (8) are enriched in euchromatic histone markers compared to the promoters of the immediate-early (IE) ICP0, ICP4, and ICP27 genes (1, 13, 21, 24, 31).