The VP22 protein is a major component of the amorphous tegument region of the Herpes Simplex Virus type I (HSV-1). Vaccine Immune Response Type: VO_0003057 Immune Response: The IgG response in serum in vaccinated mice with pVAX–HSV–2gD and pVAX–HSV–2gD–Hsp70 differed significantly (P < 0.05) from those in immunized mice with pVAX and pVAX–Hsp70, which did not generate antibody levels above background. Host Strain: BALB/c Vaccination Protocol: Each immunization of 6–8-week-old female Balb/c mice consisted of a single PMED™ delivery to the ventral abdominal skin in which each delivery contained 0.5 mg of gold coated with a total of 0.5 μg of the DNA vaccine-DEI vector formulation. Efficacy: The majority of mice immunized with plasmid DNA encoding ICP 27 resisted challenge with 10 ID50 of virus (Manickan et al., 1997). Specifically, CD40L drives immune responses toward a Th1 phenotype (Sin et al., 2001). In addition, all of the three non-structural proteins elicited a good humoral response (with titres ranging from 1:16,000 to 1:128,000). In conclusion, id administration of pgD-E7E6E5 significantly enhanced the immunogenicity and anti-tumor effects of the DNA vaccine, representing a promising administration route for future clinical trials. One major interest in the commercial development of DNA vaccines, especially for developing countries, is to increase DNA vaccine stability at room temperature, to reduce the requirement for costly cold storage, and to extend product shelf-life. Osorio et al., 2004: Osorio Y, Cohen J, Ghiasi H. In the present study, we report the immune responses and anti-tumor effects of a DNA vaccine (pgD-E7E6E5) expressing three proteins (E7, E6, and E5) of the human papillomavirus type 16 genetically fused to the glycoprotein D of the human herpes simplex virus type 1, which was administered to mice by the intradermal (id) route using a gene gun. In this study, we detected the transcripts of UL24 and LTB-UL24 genes by RT-PCR in vivo, indicating that both SL7207 (pVAX-UL24) and SL7207 (pVAX-LTB-UL24) can be expressed by APC (Figure 2). 2004; 45(2); 506-514. [PubMed: 14744892].