Measurements of hepatitis B virus (HBV) DNA levels are routinely used to identify infectious, chronic carriers and to predict and monitor efficacies of antiviral treatment regimens (1–4). Be calm while our lab experts will take you through the process safely and painlessly deliver superior health solutions with unmatched convenience and affordability. Using a pool of negative cerebrospinal fluid (CSF) samples spiked with either calibrated controls for HSV-1 and VZV or dilutions of a clinical strain that was previously quantified for HSV-2, the empirical limit of detection of the BD Max assay was 195.65, 91.80, and 414.07 copies/ml for HSV-1, HSV-2, and VZV, respectively. Aliquots tested at the reference laboratory were processed using a MagNA Pure LC DNA extractor (Roche Molecular Systems, Alameda, CA) and tested by the Roche HSV real-time PCR assay. The real-time assay agreed with our reference method for 319 out of the 323 samples tested (99%). Conclusion : Our results suggest that the real-time PCR assay was more sensitive compared with the conventional PCR. The Real-Time PCR assay was performed with the primer Design TM genesis for herpes simplex virus (Primer Design, UK).
and Hagley Ave., Christchurch, New Zealand. In comparing LAMP to real-time PCR, viral DNA was detected by the LAMP method in 9 of 12 HSV-1 DNA positive samples and 11 of 18 HSV-2 DNA positive samples. PCR using extracted and unextracted specimens was also compared to cell culture as a means of detecting HSV.